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INDONESIA
Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 6 Documents
 1 
Search results for , issue "Vol 5 No 1 (1999): December 1999" : 6 Documents clear
PENGARUH PEMBERIAN OKSITOSIN DAUN KATU DAN DAUN LAMPES TERHADAP SEKRESI AIR SUSU DAN GAMBARAN HISTOLOGI KELENJAR AMBING PADA MENCIT I.B. Rai Pidada
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 5 No 1 (1999): December 1999
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/523

Abstract

A comparative study on the effect of oxytocin, extracted leaf of katu (Sauropus androgynous, Merr) and lampes (Ocimum sanctum, Linn) against secretions of milk and histologically mammary gland of mice was carried out under laboratory conditions. The study was designed by using totally 40 mice pp and divided into four groups treatment which consisted 10 females mice post partum (pp) of each group. The control group, katu (S.androginus), lampes (O. sanctum), and oxytosin were given orally 0.5 ml physiological solution, 10 mg/0.5 ml leaf extract of S. androgynus , 10 mg/0.5 ml leaf extract of O. sanctum and 0.1 IU oxytocin intramuscular, respectively. The treatment were started on day fourt to 21th of lactation period. The data were observed on day 6th, 9th, 12th, 15th, of lactation period and than on day 21th the mice were killed to prepare histologically of mammary glands. The data analyzed by ANOVA and LSD test. The result of the study showed that the secretion of the milk on the mice were not significantly different among of fourth groups. However between S. androgynous and O. sanctum group accured an increasing on secretion of milk productions. The diameter of alveolus pit of mammae lobus gland were not significantly different amongs the fourth group, but the number of alveolus of mammae lobus gland were significantly different among oxytocin, S. androgynous, and O. sanctum group and between the group of O. sanctum and control.
PENGARUH EKSTRAK AKAR GINGSENG JAWA DAN GINGSENG KOREA TERHADAP SINTESIS PROTEIN TESTIS YANG BERPERAN DALAM SPERMATOGENESIS Dwi Winarni
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 5 No 1 (1999): December 1999
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/524

Abstract

This study was aimed to know the effect of the Jawa ginseng and Korean ginseng roots on synthesis of testicular proteins that needed for spermatogenesis. Eighteen male rats (8-9 weeks) were divided into 4 groups. Rats were given orally with ethinylestradiol (6 rats-group I), ethinylestradiol and extract of Korean ginseng root (3 rats-group II), ethinylestradiol and extract of Java ginseng (3 rats-group III), and drinking water only (6 rats-group IV). Ethinylestradiol that administrated equal to 1 mg/100 g body weight/day for each rat but extract of Java ginseng or Korean ginseng roots was equal with 3.5 mg dry ginseng root / 100 BW/each day. That treatment was done for 14 days. The testes was collected on the last day of treatment. Testes from three rats each from group I and group IV were sectioned by paraffin method and stain with hematoxylin-eosin but other testes (3 rats for each group) were made to homogenate. Testicular extract was made from I testicular homogenate : 3 phosphate buffer saline. Testicular proteins were separated by 10% SDS-PAGE. Gel was stained with Coomasie brilliant blue 0.25%. According to protein bands in gel and histological observation, this study revealed that the administration of extract of both Java ginseng and Korean ginseng roots equal with 3.5 mg dry ginseng root/ 100 BW/ day for 14 days, influence synthesis of testicular protein that have role on meiotic stage of spermatogenesis. These proteins have MW 14.44 and 68 kDa.
DETEKSI Streptococcus Sp PADA Clarias gariepinus MENGGUNAKAN AMPLIFIED ENZYME-LINKED IMMUNOSORBENT ASSAY Sri P. Astuti Wahyuningsih; R. Warsito; Hastari Wuryastuti; Kamiso H.N.
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 5 No 1 (1999): December 1999
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/525

Abstract

An enzyme-linked immunosorbent assay has been developed which detect Streptococcus sp. Twenty Clarian gariepinus at the age of two weeks was soaked in Streptococcus sp. suspension with concentration of 108 bacteria per ml for two hours. Specimens such as blood, mucous, muscles, heart, kidney, liver, intestines and gill were collected, and assayed for the presence of Steptococcus sp. using ELISA. Result of present study showed that ELISA can be applied to detect an isolated Streptococcus sp. from tissues of Clarias gariepinus. Kidney is a primary target organ (predilection). Therefore, the kidney is a specimen of choice for diagnosis approach (es) of Streptococcus sp. infection.
OVARIAN FOLLICULAR DYNAMICS IN Gn-RH ANTAGONIST TREATED HEIFERS DURING THE PERIOVULATORY PERIOD Bambang Poernomo S.
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 5 No 1 (1999): December 1999
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/526

Abstract

It is not clear wheter the turnover of ovaries follicles during the periovulatory period in Gn-RH treated heifers. This study was performed to test the hypothesis that an increase dosage of Gn-RH antagonist influences the follicular development. Six normocyclic black pied heafers was derived into equally two control and treatment groups. The injection of Gn-RH antagonist Antarelix ® (Eurpeptides, France) and vehicle (mannitol) were performed six times every 12 hours on the day-1, 0, 1 and 2, respectively. Ultrasound examinations of the ovaries can carried out using B-mode scanner and performed every day beginning from day 0. Result of the study suggest all animals showed sign of behaviorial estrous begining around 52 hours after induction of luteolysis. All control animal showed the development of a large (more than eight mm diameter) preovulatory follicle with maximum diameters between 11 and 15 mm and thereafter ovulated. Two of there controls ovulated between days 1 and 2 the remining control heifer showed ovulation between days 2 and 3. Treated animals developed large follicles with maximum diameter between 12 and 17 mm but they did not ovulate. Concerning the number of small (one to less than four mm diameter) and medium (four to eight mm diameter) follicles there were no significantly differences found between control and treated animals. However, Antarelix ® did not inhibit the development of all follicles. Antarelix ® also did not inhibit the development fo large follicles but inhibited ovulations.
PENGARUH MEDROKSI PROGESTERON ASETAT TERHADAP ULTRASTRUKTUR SEL LEYDIG TIKUS PUTIH (Rattus norvegicus) Sarmanu
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 5 No 1 (1999): December 1999
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/527

Abstract

The objective of the research was to study the effect of medroxy progesterone acetate on the ultrastructure of Leydig cells of Rattus norvegicus. Randomized complete design was applied to study. Treatment consisted of 4 doses medroxy progesterone acetate were 0, 4, 8 and 12 mg per rat respectively and 8 replications. Injection were applied perform 4 times per rat within 4 weeks interval. The testicle were examined ultrastructure change on the nucleus, endoplasmic reticulum, mitochondria and golgy apparatus of the Leydig cells. Results indicated that injections of medroxy progesterone acetate of 8 and 12 mg per rat causes significant untrastructure change of nucleus, endoplasmic reticulum, Golgi apparatus and mitochondria of Leydig cells in comparison with doses 0 and 4 mg per rat. In additions, there were no significant different in ultrastructure of Leydig cells between doses 8 and 12 mg per rat as well as there were no significant different between doses of 4 and 0 mg per rat.
TOKSISITAS LARVASIDA FRAKSI POLAR DAN NON POLAR HERBA Eclipta alba Hassk TERHADAP LARVA NYAMUK Aedes aegypti Linn Hamidah; Rosmanida; Darmawati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 5 No 1 (1999): December 1999
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/528

Abstract

Dengue Haemorrhagic Fever is a disease caused by virus and spread out by mosquitoes. Until now Aedes aegypti is thought as important vector in spreading procces of this disease. The general technique with synthetic chemical insecticide used to control density of mosquito population but this way causes the resistence of insect target, kill the untarget insect, and disturbs the environment quality. Due this problem, insecticide from plant is one of alternative such as Eclipta alba (Urang aring). The research aimed to distinguish toxicity larvacida degree between the polar fraction and non polar herba Eclipta alba to the growth of mosquito larva of Aedes aegypti. The experimental method applied with the complete random design used in this research. Each treatment to mosquito larvae of Aedes aegypti had replications and the data was analyzed of Variance (ANOVA). The result showed that non polar fraction has the higher toxicity effect compared with the polar fraction. The concentration of LC non polar fraction 319.1826 ppm50, and concentration LC50 polar fraction is 1033.888 ppm and instar larva change of non polar fraction give higher elimination than polar fraction, non polar is 24% from population has change into IV instar and non polar 45%.

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